Skip to main content
WCS
Menu
Library
Library Catalog
eJournals & eBooks
WCS Research
Archives
Research Use
Finding Aids
Digital Collections
WCS History
WCS Research
Research Publications
Science Data
Services for WCS Researchers
Archives Shop
Bronx Zoo
Department of Tropical Research
Browse By Product
About Us
FAQs
Intern or Volunteer
Staff
Donate
Search WCS.org
Search
search
Popular Search Terms
WCS History
Library and Archives
Library and Archives Menu
Library
Archives
WCS Research
Archives Shop
About Us
Donate
en
fr
Title
Development of clade-specific Symbiodinium primers for quantitative PCR (qPCR) and their application to detecting clade D symbionts in Caribbean corals
Author(s)
Correa A.M.S., McDonald M.D., Baker A.C.
Published
2009
Publisher
Marine Biology
Published Version DOI
https://doi.org/10.1007/s00227-009-1263-5
Abstract
We developed quantitative PCR (qPCR) assays to distinguish each of the four clades (A-D) of dinoflagellate endosymbionts (genus Symbiodinium) commonly found in Caribbean corals. We applied these primer sets, which target portions of the multi-copy ribosomal DNA (rDNA) gene family, to assess the presence/absence of symbionts in clade D (as indicated by the detection of clade D DNA). We detected these symbionts in five of six Caribbean host species/genera (21% of samples analyzed, N = 10 of 47 colonies), from which clade D had rarely or never been observed. This suggests that Symbiodinium in clade D are present in a higher diversity of coral species than previously thought. This qPCR-based approach can improve our understanding of the total microbial diversity associated with corals, particularly in hosts thought to be relatively specific, and has many other potential applications for studies of coral reef ecology and conservation. © 2009 Springer-Verlag.
Access Full Text
A full-text copy of this article may be available. Please email the
WCS Library
to request.
Back
PUB11879