Title
Molecular cytogenetic assignment of genes to bovine chromosome 5
Author(s)
De Donato M., Gallagher Jr. D.S., Lehn C., Gill C., Taylor J.F.
Published
2003
Publisher
Genetics and Molecular Research
Abstract
Seven genes were assigned by molecular cytogenetic methods to bovine chromosome 5. To accomplish this, specific primers were either publicly available or were designed from highly conserved regions of the publicly available mammalian gene sequences. The identity of the amplified segments was verified by sequencing and alignment with the published sequences. The optimized primers that amplified the desired bovine genes were used for screening a bovine bacterial artificial chromosome library. The positive clones were localized to a specific band of bovine chromosome 5 by fiuorescence in situ hybridization. The genes HOXC4, SP1 and IGFBP6 were localized to band q21, COL2A1 was localized to bands q21-q23, IGF1 was localized to band q26, MB to band q31 and the gene CYP2D6 was localized to band q35. The cytogenetic assignment of SP1, IGFBP6, COL2A1, IGF1, MB and CYP2D6 is first reported here and the assignment of HOXC4 refnes the previous assignment of this gene. The idenfification and localization of these genes further support the development of the human to bovine comparative map through characterizing the homologous segments conserved in the evolution of these species. This information will be useful for the future localization of genes that affect economically important traits in bovines.
Keywords
cytochrome P450 2D6; homeodomain protein; myoglobin; primer DNA; somatomedin binding protein; somatomedin binding protein 6; somatomedin C; transcription factor Sp1; article; bacterial artificial chromosome; cattle; chromosome 5; chromosome band; clone; cytogenetics; eukaryote evolution; fluorescence in situ hybridization; gene assignment; gene identification; gene library; gene location; gene sequence; genetic conservation; human; human versus animal comparison; mammalian genetics; molecular genetics; nonhuman; sequence alignment; sequence analysis; sequence characterized amplified region; animal; animal disease; chromosome map; comparative study; DNA sequence; genetics; methodology; polymerase chain reaction; quantitative trait; Animalia; Bacteria (microorganisms); Bos taurus; Bovinae; Eukaryota; Mammalia; Animals; Cattle; Chromosome Mapping; Chromosomes, Artificial, Bacterial; Humans; In Situ Hybridization, Fluorescence; Polymerase Chain Reaction; Quantitative Trait, Heritable; Sequence Analysis, DNA

Access Full Text

A full-text copy of this article may be available. Please email the WCS Library to request.



Back

PUB12464