Skip to main content
WCS
Menu
Library
Library Catalog
eJournals & eBooks
WCS Research
Archives
Research Use
Finding Aids
Digital Collections
WCS History
WCS Research
Research Publications
Science Data
Services for WCS Researchers
Archives Shop
Bronx Zoo
Department of Tropical Research
Browse By Product
About Us
FAQs
Intern or Volunteer
Staff
Donate
Search WCS.org
Search
search
Popular Search Terms
WCS History
Library and Archives
Library and Archives Menu
Library
Archives
WCS Research
Archives Shop
About Us
Donate
en
fr
Title
Efficient generation of vesicular stomatitis virus (VSV)-pseudotypes bearing morbilliviral glycoproteins and their use in quantifying virus neutralising antibodies
Author(s)
Logan, N.;McMonagle, E.;Drew, A. A.;Takahashi, E.;McDonald, M.;Baron, M. D.;Gilbert, M.;Cleaveland, S.;Haydon, D. T.;Hosie, M. J.;Willett, B. J.
Published
2016
Publisher
Vaccine
Published Version DOI
https://doi.org/10.1016/j.vaccine.2015.12.006
Abstract
Morbillivirus neutralising antibodies are traditionally measured using either plaque reduction neutralisation tests (PRNTs) or live virus microneutralisation tests (micro-NTs). While both test formats provide a reliable assessment of the strength and specificity of the humoral response, they are restricted by the limited number of viral strains that can be studied and often present significant biological safety concerns to the operator. In this study, we describe the adaptation of a replication-defective vesicular stomatitis virus (VSV Delta G) based pseudotyping system for the measurement of morbillivirus neutralising antibodies. By expressing the haemagglutinin (H) and fusion (F) proteins of canine distemper virus (CDV) on VSV Delta G pseudotypes bearing a luciferase marker gene, neutralising antibody titres could be measured rapidly and with high sensitivity. Further, by exchanging the glycoprotein expression construct, responses against distinct viral strains or species may be measured. Using this technique, we demonstrate cross neutralisation between CDV and peste des petits ruminants virus (PPRV). As an example of the value of the technique, we demonstrate that UK dogs vary in the breadth of immunity induced by CDV vaccination; in some dogs the neutralising response is CDV-specific while, in others, the neutralising response extends to the ruminant morbillivirus PPRV. This technique will facilitate a comprehensive comparison of cross-neutralisation to be conducted across the morbilliviruses. (C) 2015 The Authors. Published by Elsevier Ltd.
Keywords
Morbillivirus;Neutralisation;CDV;PPRV;Pseudotype;canine-distemper virus;petits ruminants virus;epithelial-cell;receptor;measles-virus;hemagglutinin protein;stable transduction;natural infection;lentiviral vector;rhesus-monkeys;slam cd150;Immunology;Research & Experimental Medicine
Access Full Text
A full-text copy of this article may be available. Please email the
WCS Library
to request.
Back
PUB18959