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Title
Foot and mouth disease and brucellosis serological survey in mixed herds of camelids and sheep in the highlands of Apolobamba Eco-Region, La Paz - Bolivia / Estudio serológico de fiebre aftosa y brucelosis en rebaños mixtos de camélidos y ovinos en la ecorregión de serranía en Apolobamba, La Paz - Bolivia
Author(s)
L. Fabián Beltrán-Saavedra S.; Herminio Ticona; Rodolfo Nallar G.; José Luis Gonzáles R.
Published
2010
Publisher
Revista de Investigaciones Veterinarias del Perú
Abstract
The objective of the present study was to determine serological levels of antibodies against foot and mouth disease (FMD) and brucellosis in mixed herds of alpacas and sheep in four communities of the highland ecorregion of the Apolobamba Protected Area (National Integrated Management Natural Area - ANMIN), La Paz - Bolivia, bordering with Peru. Blood samples of 99 alpacas and 42 sheep of different sex and ages were collected. Plasma samples were tested for the detection of VIA antibodies against FMD virus using the agar gel immunodiffusion test (AGID-VIA) and for the detection of antibodies against smooth strains of Brucella spp. using a plate agglutination test as screening and a competitive ELISA (c-ELISA) as a confirmatory test. The results of the study showed no seropositive reactors for FMD or brucellosis. / El objetivo del presente estudio fue determinar niveles serológicos de anticuerpos contra fiebre aftosa y brucelosis en rebaños mixtos de alpacas y ovejas en cuatro comunidades de la ecorregión serranía en el Área Natural de Manejo Integrado Nacional Apolobamba, La Paz - Bolivia, colindante con Perú. Se colectaron 99 muestras de sangre de alpacas y 42 de ovinos de ambos sexos y edad diversa. Las muestras se procesaron mediante la prueba de inmunodifusión en gel agar para detectar anticuerpos VIA contra el virus de la fiebre aftosa y las pruebas de Aglutinación Rápida en Placa (prueba tamiz) y ELISA de competición (c-ELISA) (prueba confirmativa) para la detección de anticuerpos contra cepas lisas de Brucella sp. No se detectó anticuerpos contra fiebre aftosa o brucelosis.
Keywords
foot and mouth disease; Brucella spp; alpaca; camelid; ovine; serology; Apolobamba; fiebre aftosa; camélido; ovinos; serología
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PUB35756